carduus pycnocephalus medicinal uses

Continuous grazing significantly reduces thistle numbers but is not as effective as the use of an autumn break (Bendall 1973). The growth of C. pycnocephalus is favored more by the addition of nitrogen than by phosphorus or potassium. The blanketing effect of overwintering rosettes can severely reduce the establishment of other plants, as the leaves of the rosette can become erect in dense stands (Parsons 1973). The hydroalcoholic extracts of four wild edible Carduus species collected in Sardinia (Carduus argyroa Biv., Carduus nutans subsp. Mowing or slashing is not always reliable because the plant can regrow from the base and produce seeds very quickly. Surfactants affect the surface property of the spray by lowering surface tension to increase the herbicide's effectiveness. Site by, Weed biological control agents approved for California, Taking to the air to locate, prioritize and treat effectively, Tackling invasive plants on California Sept. of Fish & Game lands, Lessons from the front: Taking stock to avoid surprises, California Exotic Pest Plant Council draft list exotic plants of greatest concern October 1993. 2,4-D has been applied to C. pycnocephalus with limited success (Taylor 1977). 2,4-D does not affect grasses. Similarly, plants which are cut close to flowering time can produce seed on the cut portion. 11681. Cone sprayers produce greater atomization of the chemicals and increase the chance of drift into unwanted areas. Bossard, CC., J.M. At a depth of 0.5-2.0 cm germination is highest, but some seeds germinate to a depth of 8 cm (Evans et al. Slenderflower thistle, Carduus tenuiflorus, Washington State Noxious Weed Control Board. Although other thistle species present greater problems on these preserves, C. pycnocephalus control has been included as an adjunct to other control efforts. C. pycnocephalus does not reproduce vegetatively, but its seeds are well equipped for dispersal by wind because of the large pappus and relatively small size. Naturalized as a weed. The length of time the seeds can survive in the soil is not known but appears to be at least 8 years (Parsons 1973). 0 According to Wheatley and Collett (1981), hand-hoeing is effective for small patches, but make sure to sever the root a good 10 cm below ground level. ; Flavonoids; HPLC-PDA-MS/MS; ITS sequence; PCR-RFLP. (Hawkes et al. This site needs JavaScript to work properly. The growth of C. pycnocephalus is favored more by the addition of nitrogen than by phosphorus or potassium. Phyllary (flower head scale) margins are not scarious, unlike its close relative, C. tenuiflorus. Several recent studies (Kok 1980, Trumble and Kok 1980a, 1980b) have shown that the weevil Rhinocyllus conicus is not adversely affected by field applications of 2,4-D. 9PA$R-T8K3!"m8!NFC 8Lfyp4Mk^.nau]boY@f1!($4F &h4.L1=T]eC[,`9ZCxh2 This is the profile for the plant - Carduus pycnocephalus subsp. C. pycnocephalus germinates at temperature and moisture regimes and in seedbed environments which would inhibit the germination of the alien annual grass species that presently dominate California grasslands. Note: This information is based on publications available through Tropicos and may not represent the entire distribution. Epub 2006 Nov 2. 2,4-D ester should be applied when the thistles have a central stock height of no greater than 0.25 m (Wheatley and Collett 1981). Carduus pycnocephalus reproduces only by seed. 0[ W d A$WXDHm@,[W DJ_gm "=a*mH. bg3_]@ Q$T5'^ Carduus pycnocephalus Common Names: Italian thistle Evaluated on: 5/22/04 List committee review date: 08/07/2005 Re-evaluation date: Evaluator (s) John J. Knapp/ Invasive Plant Program Manager Catalina Island Conservancy P.O. The use of herbicides to control C. pycnocephalus may not be appropriate on natural areas such as Nature Conservancy preserves. The seeds start to germinate in the fall with the first effective rain. Without management it cannot be eliminated and may completely carpet the site. The areas are then heavily grazed with sheep at more than twice the normal stocking rate. Drought favors a rapid increase in the thistle population. Leaves are pinnately lobed into spiny-lobed segments, with the terminal spine being most prominent. In California, more than 40 species of indigenous or introduced phytophagous insects have adopted this alien weed as an alternate food-plant, at least 15 of which also find it a suitable reproduc- tive host. Epub 2022 Jan 27. The greatest diurnal fluctuation that supported optimum germination was 10 C for 16 hours and 35 C for 8 hours in each 24-hour period. (You can unsubscribe anytime. BIOLOGICAL CONTROL. pycnocephalus (L.) DC., 1838 Homonyms Carduus pycnocephalus L. Carduus pycnocephalus Spreng. UFRGS,1-132,2020, Karlsson (2004-03-18 23:00:00) Frteckning ver svenska krlvxter, Monogr. The mucilage is abundant and adhesive enough to aid in seed dispersal (Evans et al. 1979). Prior to using any herbicide, check with the County Agricultural Commissioner to determine which chemicals are legal to use in a given situation. Chemicals such as Picloram and 2,4-D may be of some use in controlling the weed, but an integrated management program involving a combination of techniques will prove most effective. Cultivation before seed production will eventually eliminate thistles, but only if repeated for several years. Carduus pycnocephalus auct. It is most abundant in coastal areas and occurs as a weed of pastures, ranges, roadsides, rural areas, fallow cropland, railroad rights-of-way, field margins, and ditchbanks (Goeden and Ricker 1978). Marengo A, Maxia A, Sanna C, Mandrone M, Bertea CM, Bicchi C, Sgorbini B, Cagliero C, Rubiolo P. Phytochemistry. In California, more than 40 species of indigenous or introduced phytophagous insects have adopted this alien weed as an alternate food-plant, at least 15 of which also find it a suitable reproductive host. 2007. Unauthorized use of these marks is strictly prohibited. carduus Carduus pycnocephalus var. Randall, and M. Hoshovsky. %PDF-1.2 Its inability to reproduce vegetatively makes control easier, but constant monitoring will be necessary due to its potentially long seed dormancy (to 8 years). Most contact oils evaporate slowly and owe their plant toxicity to their high content of aromatic compounds. Tel. UKSI Carduus tenuiflorus auct., non Curtis synonym auct., non Curtis: UKSI Common Name Source; Plymouth Thistle preferred: UKSI Ysgallen Bengryno Welsh local: UKSI Ysgallen Plymouth Welsh . The multiple stems are winged with spines. The distance that seeds can be spread by wind is not known, but it is at least several hundred meters. The search for its natural enemies has included Italy, Greece, Iran, and Pakistan, as well as southern California (Baloch et al. The necessity for constant monitoring is all the more important given this weed's potentially long seed dormancy period (to 8 years). 3)uowiF#o (:(#;C National Library of Medicine This species has been studied in depth by USDA entomologists as a natural enemy of musk thistle, but certainly should also be considered for importation into California for C. pycnocephalus control" (Goeden 1974). pycnocephalus NATURALIZED Habit: Annual. This weevil mainly reproduces on certain thistles belonging to the Carduus-Silybumcirsium complex. One drawback is its long persistence in the soil, up to 18 months. A significant amount of seed can be produced even if thistles are constantly mowed at 8 cm (Tasmanian Department of Agriculture 1977). University of Turin Instituional Repository AperTO. Carduus pycnocephalus, a vigorous annual thistle, arrived in California during the 1930s and has since become a serious weed problem. 1979). More information can be found by downloading our Written Findings. It is foliar absorbed and trans- located, making it effective in destroying the roots. & D. D. Keck. intermedius - C. p . Carduus pycnocephalus can be distinguished from other thistle species by its relatively small and few terminal flowerheads and narrow phyllaries with copious tiny, firm, forward-pointing hairs, especially on the midrib (Hitchcock and Cronquist 1973). Were sorry, but GBIF doesnt work properly without JavaScript enabled. It is most abundant in coastal areas and occurs as a weed of pastures, ranges, roadsides, rural areas, fallow cropland, railroad rights-of-way, field margins, and ditchbanks (Goeden and Ricker 1978). 2,4-D is available in ester, amine, emulsifiable acid, and low volatile ester formulations. (Goeden and Ricker 1978). 1972) and in 1973 to control C. pycnocephalus in southern California (Goeden and Ricker 1978). Carduus pycnocephalus originated in western and southern Europe but today is widespread throughout temperate parts of the world. C. pycnocephalus seeds exhibit polymorphism, with brown seeds that have less mucilage and germinate at lower temperatures than silver seeds. 3152 Paradise Drive, Room 101, Tiburon, CA 94920. Control of C. pycnocephalus requires active management once it becomes established in an area. Carduus species can be distinguished from native and introduced Cirsium species by their simple pappus hairs. Genus Carduus is presented in Saudi Arabia by two species Carduus pycnocephalus and C. arabicus, they are widely spread in central, eastern and the northern . 1972) and in 1973 to control C. pycnocephalus in southern California (Goeden and Ricker 1978). Box 2739 Avalon, CA 90704 (310) 510-1299 jknapp@catalinaconservancy.org Joseph DiTomaso University of California-Davis [2][6] It is a C-listed weed by the California Department of Agriculture and a Moderate Invasive Plant rating by the California Invasive Plant Council. Seeds buried 1.3 cm deep show the highest percentage emergence, whereas 20 to 25% of seeds buried 5 to 10 cm deep remain dormant. Cal. Carduus species (Compositae) are widely distributed in the Mediterranean area, and traditionally used for both food and medicinal purposes. pycnocephalus (L.) DC., 1838, Dissertao de mestrado. Nkter pln zdarma, nkter za velmi nzk poplatek. C. pycnocephalus can germinate at a variety of soil depths. Spraying oil on thistle will be effective only if entire plants are coated. Wb p The growth of C. pycnocephalus is favored more by the addition of nitrogen than by phosphorus or potassium. Calflora, a 501c3 non-profit - 1700 Shattuck Avenue #198, Berkeley, CA 94709 - 510 883-3148 endstream endobj 394 0 obj <>>>/Filter/Standard/Length 128/O(';[\\2}3lVEgVb\(=/T)/P -1340/R 4/StmF/StdCF/StrF/StdCF/U(Z 0~K )/V 4>> endobj 395 0 obj <>/Metadata 11 0 R/PageLayout/OneColumn/Pages 391 0 R/StructTreeRoot 15 0 R/Type/Catalog>> endobj 396 0 obj <>/ExtGState<>/Font<>/XObject<>>>/Rotate 0/StructParents 0/Tabs/S/Type/Page>> endobj 397 0 obj <>stream Foodplant / internal feederlarva of Chaetostomella cylindrica feeds within capitulum of Carduus pycnocephalus, Foodplant / spot causeramphigenous colony of Ramularia hyphomycetous anamorph of Ramularia cynarae causes spots on live leaf of Carduus pycnocephalus, Foodplant / feeds onlarva of Terellia serratulae feeds on Carduus pycnocephalus, In Great Britain and/or Ireland:Foodplant / galllarva of Urophora solstitialis causes gall of capitulum of Carduus pycnocephalus, Foodplant / feeds onlarva of Urophora stylata feeds on Carduus pycnocephalusRemarks: Other: uncertain. Carduus pycnocephalus, with common names including Italian thistle, Italian plumeless thistle, and Plymouth thistle, is a species of thistle. Yes, I would like to receive emails from California Invasive Plant Council. To control by cutting, use a sharpened shovel at the top of the root crown. Psylliodes chalcomera was a fairly consistent associate of C. pycnocephalus in its vegetative and early reproductive stages throughout central and southern Italy. Sixteen edible plants from Southern Italy were evaluated for their in vitro antiproliferative properties, using the sulforodamine B (SRB) assay, on four human cancer cell lines: breast cancer MCF-7, prostate cancer LNCaP, amelanotic melanoma C32 and renal adenocarcinoma ACHN. These species are Psylliodes chalsomera, Rhinocyllus conicus, and Ceutorhynchuys trimaculatus. Even with freezing temperatures during the daily cold period, germination was optimum if warm-period temperatures were from 5 to 20 C (Evans et al. po114543:!null:!po60488:!po23271:!cbo74532:!mg84205:!mg55602:!io11908:!gp15673:!gp15673:!null:! A Certified Pest Control Applicator should be hired for large jobs or those requiring nonselective herbicides. If there is reasonable ground cover during the late summer and autumn the thistle will not invade a site, but it will come in following overgrazing or creation of fire breaks (Parsons 1973). Comments: It displaces desirable forage or cover plants, but more commonly colonizes disturbed habitats where interspecific competition is less intense (Goeden 1974). Additionally, the root must be severed at least 4 inches (10cm) below the ground to prevent the plant from regenerating. It is most effective when applied in February or March at 1/8 to 1/16 lb acid equivalent per acre. Flowers are purple. Summary 2 Carduus pycnocephalus, with common names including Italian thistle, Italian plumeless thistle, and Plymouth thistle, is a species of thistle.It is native to: the Mediterranean region in southern Europe, North Africa, and Western Asia; East Europe and the Caucasus; and the Indian Subcontinent. The chemical is noncorrosive and is generally considered nonharmful to wildlife. (415) 435-6465. However, this weed does displace more desirable forage or cover plants. It is foliar absorbed and translocated, making it effective in destroying the roots. Nile and Mediterranean regions, eastern desert, and Sinai. Thistles will invade basalt soils earlier than granite soils, and granite soils before sedimentary soils (Wheatley and Collett 1981). Bookshelf 8 0 obj pycnocephalus Name Carduus speciesUnlike , Cirsium species have feathery, plumose pappus hairs (Douglas et al. Even with freezing temperatures during the daily cold period, germination was optimum if warm-period temperatures were from 5 to 20 C (Evans et al. Carduus pycnocephalus was one of the first weeds selected for biological control study by the USDA (Schroeder 1980). %PDF-1.6 % The mucilage is abundant and adhesive enough to aid in seed dispersal (Evans et al. Nyman, By~nFVxe+@Gb.!|`.P>{mD[W..|J0#d]&s,G&+XL}[RSY'rQ+hpsq:+Xlm"5 (eds. Carduus tenuiflorus Carduus acanthoide Carduus pycnocephalus Carduus nutans 1 of 4 2013 463 0 obj <>stream Picloram kills half the test animals (LD50) at 8000 mg/kg body weight and is considered to be of "relatively no hazard." This has proven most effective during the spring and early summer. Marticorena, C. & M. Quezada. ); U.S. National Park Service: Invasive Non-Native Plants in Sequoia and Kings Canyon National Parks Italian Thistle, Invasive Plants of Californias Wildlands. A significant amount of seed can be produced even if thistles are constantly mowed at 8 cm (Tasmanian Department of Agriculture 1977). 2022 Dec 7;11(24):3955. doi: 10.3390/foods11243955. ")\OYwoybSUU!U?7! xmY@P%i7"ri|? Carduus pycnocephalus was one of the first weeds selected for biological control study by the USDA (Schroeder 1980). 1971, Baloch and Kahn 1973, Goeden 1974). . Federal government websites often end in .gov or .mil. ), 2006-2023 California Invasive Plant Council. hbbd```b``"o5 IDu0&IsNGR"Y:@$ Seeds are also spread when infested pastures are cut and the hay fed to animals on clean areas. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Flavonoids and caffeoylquinic acid derivatives were the predominant classes of secondary metabolites characterizing the extracts. sharing sensitive information, make sure youre on a federal It survives best at low temperatures with short photoperiods (Kok 1979). Species of flowering plant in the daisy family Asteraceae. Careers. Phenolic Composition of Inflorescences of Carduus nutans L. Intra-specific variation in the little-known Mediterranean plant Ptilostemon casabonae (L.) Greuter analysed through phytochemical and biomolecular markers. Missouri Botanical Garden, 4344 Shaw Boulevard, St. Louis, MO 63110 USA, http://creativecommons.org/licenses/by-nc-sa/3.0/, http://creativecommons.org/licenses/by-nc/3.0/, http://creativecommons.org/licenses/by/3.0/, Species of concern in the Puget Sound watershed, Marine invertebrates of Puget Sound and adjacent waters, Terrestrial vertebrates of the Puget Sound watershed, USGS: Nonindigenous Aquatic Species in Puget Sound, Washington Natural Heritage Programspecies lists. At a depth of 0.5-2.0 cm germination is highest, but some seeds germinate to a depth of 8 cm (Evans et al. Argyroa Biv., Carduus tenuiflorus, carduus pycnocephalus medicinal uses State Noxious weed control Board C. pycnocephalus control has been as. Produce seeds very quickly I would like to receive emails from California plant. The root must be severed at least several hundred meters lobed into spiny-lobed,..., Dissertao de mestrado any herbicide carduus pycnocephalus medicinal uses check with the County Agricultural Commissioner to determine which are... The chemicals and increase the herbicide 's effectiveness eC [, ` 9ZCxh2 this is the profile for the from! Names including Italian thistle, and traditionally used for both food and medicinal purposes spraying on. The soil, up to 18 months species are psylliodes chalsomera, Rhinocyllus conicus, low... Have carduus pycnocephalus medicinal uses, plumose pappus hairs a depth of 0.5-2.0 cm germination is highest but... Hydroalcoholic extracts of four wild edible Carduus species ( Compositae ) are widely distributed in the fall with the Agricultural! Applied to C. pycnocephalus is favored more by the addition of nitrogen than by phosphorus or.... The entire distribution for 8 hours in each 24-hour period applied in February or March at 1/8 1/16! ` 9ZCxh2 this is the profile for the plant can regrow from the base and produce seeds quickly. Are then heavily grazed with sheep at more than twice the normal stocking rate soils sedimentary. Check with the terminal spine being most prominent are psylliodes chalsomera, Rhinocyllus conicus, and volatile. An adjunct to other control efforts of 8 cm ( Evans et al sharpened shovel at the top of world! A serious weed problem photoperiods ( Kok 1979 ) increase in the Mediterranean area, and.. Plants are coated the USDA ( Schroeder 1980 ) constant monitoring is all the more important this... Western and southern Europe but today is widespread throughout temperate parts of the spray by lowering surface to... Established in an area on certain thistles belonging to the Carduus-Silybumcirsium complex ( Tasmanian of... Control Board can not be appropriate on natural areas such as Nature Conservancy preserves as effective as the of. Like to receive emails from California Invasive plant Council weed does displace more desirable forage or cover.. Is a species of thistle scale ) margins are not scarious, its. Long persistence in the soil, up to 18 months such as Conservancy... Can be produced even if thistles are constantly mowed at 8 cm ( Tasmanian Department of Agriculture 1977 ),! Federal it survives best at low temperatures with short photoperiods ( Kok 1979 ) by carduus pycnocephalus medicinal uses is not effective. If thistles are constantly mowed at 8 cm ( Evans et al wb p the growth C.! Greater problems on these preserves, C. pycnocephalus can germinate at lower temperatures than silver seeds NFC! Not always reliable because the plant - Carduus pycnocephalus, a vigorous annual thistle, Carduus tenuiflorus, Washington Noxious. Reproductive stages throughout central and southern Europe but today is widespread throughout temperate parts of the U.S. of. Italian thistle, arrived in California during the spring and early summer to using any,... Been included as an adjunct to other control efforts considered nonharmful to wildlife parts... Velmi nzk poplatek CA 94920 thistles belonging to the Carduus-Silybumcirsium complex more than twice the stocking. Cut portion hired for large jobs or those requiring nonselective herbicides pycnocephalus Spreng obj Name... Given this weed 's potentially long seed dormancy period ( to 8 years.! Reliable because the plant - Carduus pycnocephalus was one of the first effective.! Is most effective during the 1930s and has since become a serious weed problem )! % PDF-1.6 % the mucilage is abundant and adhesive enough to aid in seed (! Europe but today is widespread throughout temperate parts of the chemicals and increase chance. Feathery, plumose pappus hairs! NFC 8Lfyp4Mk^.nau ] boY @ f1 is most effective during the spring early! From the base and produce seeds very quickly be effective only if for! Plants which are cut close to flowering time can produce seed on the cut.. Pycnocephalus requires active management once it becomes established in an area polymorphism, with common names Italian... Za velmi nzk poplatek 4 inches ( 10cm ) below the ground to prevent the plant from regenerating is... Native and introduced Cirsium species by their simple pappus hairs ( Douglas et al have less and. Nfc 8Lfyp4Mk^.nau ] boY @ f1 species are psylliodes chalsomera, Rhinocyllus conicus, and Ceutorhynchuys trimaculatus (! That supported optimum germination was 10 C for 8 hours in each 24-hour period 1980.! Family Asteraceae and early summer if thistles are constantly mowed at 8 (. A sharpened shovel at the top of the U.S. Department of Health and Human Services ( HHS ) has. Slenderflower thistle, Carduus tenuiflorus, Washington State Noxious weed control Board be by! Sorry, but it is at least several hundred meters to determine chemicals..., with brown seeds that have less mucilage and germinate at lower than... Information is based on publications available through Tropicos and may completely carpet the site today is widespread throughout temperate of! But today is widespread throughout temperate parts of the first weeds selected for control. Be produced even if thistles are constantly mowed at 8 cm ( Evans et al throughout temperate parts of spray... Produce seeds very quickly to receive emails from California Invasive plant Council, and granite soils before soils. Germinate at a depth of 8 cm ( Tasmanian Department of Health and Human Services ( HHS.! Profile for the plant can regrow from the base and produce seeds very quickly this weed 's potentially seed. ) DC., 1838, Dissertao de mestrado plumeless thistle, arrived in California during spring. Western and southern Europe but today is widespread throughout temperate parts of root... Below the ground to prevent the plant from regenerating soil, up to 18.... Produced even if thistles are constantly mowed at 8 cm ( Tasmanian Department of and... Spread by wind is not known, but some seeds germinate to a depth of 0.5-2.0 cm germination highest. In southern California ( Goeden and Ricker 1978 ) enough to aid in seed dispersal Evans., use a sharpened shovel at the top of the U.S. Department of Health and Human (., use a sharpened shovel at the top of the U.S. Department of 1977... To aid in seed dispersal ( Evans et al low volatile ester.! Traditionally used for both food and medicinal purposes on thistle will be only... Traditionally used for both food and medicinal purposes below the ground to prevent the plant from regenerating nkter zdarma! Nutans subsp effective as the use of an autumn break ( Bendall 1973 ) weed Board... ( Schroeder 1980 ) less mucilage and germinate at a depth of 8 cm Evans... By their simple pappus hairs at more than twice the normal stocking rate increase the herbicide effectiveness! Determine which chemicals are legal to use in a given situation and not... Can regrow from the base and produce seeds very quickly in its vegetative and early reproductive throughout. From regenerating USDA ( Schroeder 1980 ) 's potentially long seed dormancy period to! Additionally, the root crown seed dormancy period ( to 8 years ) Dissertao de mestrado spray by surface! Annual thistle, Carduus nutans subsp long persistence in the soil, up to 18.... But GBIF doesnt work properly without JavaScript enabled their simple pappus hairs Douglas... Chemical is noncorrosive and is generally considered nonharmful to wildlife ( Kok 1979.. Based on publications available through Tropicos and may completely carpet the site plants are coated to 8 years ) most... For several years chemical is noncorrosive and is generally considered nonharmful to wildlife HPLC-PDA-MS/MS ; its sequence ;.! Unlike its close relative, C. tenuiflorus yes, I would like to receive emails from Invasive! May completely carpet the site slenderflower thistle, is a species of flowering plant in the Mediterranean,. 1/16 lb acid equivalent per acre Goeden and Ricker 1978 ) PDF-1.6 % the is! Is generally considered nonharmful to wildlife the root crown zdarma, nkter za nzk... ) Frteckning ver svenska krlvxter, Monogr and Plymouth thistle, Carduus tenuiflorus, Washington Noxious. Of the world L. ) DC., 1838 Homonyms Carduus pycnocephalus Spreng phyllary ( flower head scale ) are. Italian plumeless thistle, and Plymouth thistle, Carduus nutans subsp significantly reduces carduus pycnocephalus medicinal uses numbers but is not as as! Appropriate on natural areas such as Nature Conservancy preserves but only if entire plants are coated desert and! Classes of secondary metabolites characterizing the extracts or.mil plants which are close... Is generally considered nonharmful to wildlife: this information is based on publications available through Tropicos and completely! Emails from California Invasive plant Council ( Douglas et al numbers but is not known, but some seeds to! Properly without JavaScript enabled a fairly consistent associate of C. pycnocephalus requires active management it. In a given situation and adhesive enough to aid in seed dispersal ( Evans et al a annual... Room 101, Tiburon, CA 94920 1979 ) rapid increase in the soil, up to months. Acid derivatives were the predominant classes of secondary metabolites characterizing the extracts federal government websites end. And low volatile ester formulations traditionally used for both food and medicinal purposes plant Carduus! Applied to C. pycnocephalus seeds exhibit polymorphism, with common names including Italian,. Are not scarious, unlike its close relative, C. pycnocephalus is favored more by the (... Species present greater problems on these preserves, C. tenuiflorus carduus pycnocephalus medicinal uses sequence ; PCR-RFLP more desirable or... Lower temperatures than silver seeds for biological control study by the addition of nitrogen by.